Viral Vector Effects on Exoenzyme C3 Transferase–Mediated Actin Disruption and on Outflow Facility
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چکیده
منابع مشابه
Excimer laser effects on outflow facility and outflow pathway morphology.
PURPOSE To determine the relative contributions to aqueous outflow resistance of the tissues distal to the inner wall of Schlemm's canal. METHODS While performing constant pressure perfusion at 10 mm Hg, a 193-nm excimer laser (Questek) was used to precisely remove portions of sclera, unroofing Schlemm's canal while leaving the inner wall intact. The laser beam was masked to produce a beam 2 ...
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The effect of C3 transgene expression on actin and cellular adhesions in cultured human trabecular meshwork cells and on outflow facility in organ cultured monkey eyes.
PURPOSE To determine the effects of adenovirus-delivered exoenzyme C3 transferase (C3) gene expression on cultured human trabecular meshwork (HTM) cells and on outflow facility in organ cultured monkey anterior segments. METHODS An adenoviral (Ad) vector expressing both C3 and green fluorescent protein (GFP) was used to transduce cultured HTM cells. Changes in cell morphology and the organiza...
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PURPOSE In vivo, biomechanical stress plays an important role in tissue physiology and pathology, affecting cell and tissue behavior. Even though conventional outflow tissues are constantly exposed to dynamic changes in intraocular pressure, effects of such biomechanical stressors on outflow function have not been analyzed. The purpose of the present study was to determine the effect(s) of ocul...
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OBJECTIVES To determine the effects of the serine-threonine kinase inhibitor H-7 on (1) cell junctions and the attached actin-based cytoskeleton in cultured bovine aortic endothelial cells, and (2) outflow facility in living monkeys. METHODS Bovine aortic endothelial cells were cultured by standard techniques. The architecture and distribution of actin filaments, vinculin, and beta-catenin in...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2015
ISSN: 1552-5783
DOI: 10.1167/iovs.14-15909